NUMS — Medical & Dental Entry Test

NUMS Biology — Bioenergetics (Set 2)

Take the NUMS Biology — Bioenergetics (Set 2): 30 multiple-choice questions (40 minutes) with instant scoring and a full answer review. Covers 30 NUMS…

What this test covers

Sample questions with answers

The first 5 questions of this test, with the correct answer and explanation.

  1. Q1. Engelmann placed a filamentous alga under light split into a spectrum and added aerobic bacteria. The bacteria clustered mostly around regions lit by red and blue light. This showed that:

    • A. Photosynthesis (O₂ release) is fastest at wavelengths absorbed by chlorophyll ✓
    • B. Oxygen is released from CO₂
    • C. Bacteria photosynthesise in red light
    • D. Green light is used most efficiently

    Answer: A. Photosynthesis (O₂ release) is fastest at wavelengths absorbed by chlorophyll

    Explanation: Aerobic bacteria gathered where most O₂ was produced; this matched the absorption peaks of chlorophyll, giving an early action spectrum.

  2. Q2. A plant is given water containing ordinary oxygen and CO₂ labelled with ¹⁸O. Where will most of the ¹⁸O be found after photosynthesis?

    • A. In the chlorophyll molecule
    • B. Only in the released oxygen gas
    • C. In the carbohydrate and water produced, not in the released O₂ ✓
    • D. Only in ATP

    Answer: C. In the carbohydrate and water produced, not in the released O₂

    Explanation: O₂ released comes from water (photolysis); oxygen atoms from CO₂ end up in the sugar and in the water formed in the overall reaction.

  3. Q3. The overall balanced equation of photosynthesis producing one molecule of glucose uses how many molecules of CO₂?

    • A. 12
    • B. 3
    • C. 1
    • D. 6 ✓

    Answer: D. 6

    Explanation: 6CO₂ + 12H₂O → C₆H₁₂O₆ + 6O₂ + 6H₂O: six carbon atoms in glucose come from six CO₂.

  4. Q4. In paper chromatography of leaf pigments, carotene travels furthest with the solvent front. This is because carotene:

    • A. Absorbs the most light
    • B. Is the largest pigment molecule
    • C. Contains magnesium
    • D. Is the most soluble in the non-polar solvent and least attracted to the paper ✓

    Answer: D. Is the most soluble in the non-polar solvent and least attracted to the paper

    Explanation: Carotene is a hydrocarbon, very non-polar, so it moves with the solvent and has the highest Rf value.

  5. Q5. The chlorophyll molecule has a porphyrin head with a central atom of:

    • A. Iron
    • B. Magnesium ✓
    • C. Manganese
    • D. Copper

    Answer: B. Magnesium

    Explanation: Chlorophyll's porphyrin ring holds Mg²⁺; haem has Fe²⁺. Mg deficiency therefore causes chlorosis.

The other 25 questions

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  1. Q6. The reaction-centre chlorophyll of photosystem I absorbs maximally at 700 nm. If a plant is lit only with light of wavelength above 690 nm, which effect is expected?

    • A. Photolysis of water increases
    • B. Non-cyclic electron flow falls sharply because PSII (P680) is poorly excited
    • C. Only PSII is active
    • D. The Calvin cycle stops needing ATP
  2. Q7. The enzyme complex that splits water during the light reactions is associated with photosystem II and requires ions of:

    • A. Manganese
    • B. Zinc
    • C. Sodium
    • D. Cobalt
  3. Q8. A herbicide blocks electron flow from photosystem II to plastoquinone. Which immediate effect would be observed in illuminated chloroplasts?

    • A. Oxygen evolution and NADPH production both stop
    • B. The Calvin cycle speeds up
    • C. Oxygen release increases
    • D. More NADPH but no ATP is produced
  4. Q9. The final electron acceptor in non-cyclic photophosphorylation is:

    • A. NADP⁺
    • B. Plastocyanin
    • C. Oxygen
    • D. Water
  5. Q10. How many turns of the Calvin cycle are required to make one molecule of glucose?

    • A. 12
    • B. 6
    • C. 3
    • D. 2
  6. Q11. To produce one molecule of glucose, the Calvin cycle uses how many molecules of ATP and NADPH?

    • A. 18 ATP and 12 NADPH
    • B. 36 ATP and 12 NADPH
    • C. 12 ATP and 18 NADPH
    • D. 6 ATP and 6 NADPH
  7. Q12. RuBisCO, the enzyme that fixes CO₂ in the Calvin cycle, combines CO₂ with:

    • A. Ribulose bisphosphate (5C)
    • B. Phosphoenolpyruvate (3C)
    • C. Oxaloacetate (4C)
    • D. Glyceraldehyde phosphate (3C)
  8. Q13. In C₄ plants, the first stable product of CO₂ fixation in mesophyll cells is:

    • A. Pyruvate
    • B. Ribulose bisphosphate
    • C. Oxaloacetate, a 4-carbon acid
    • D. 3-phosphoglycerate
  9. Q14. On a hot, bright day with partly closed stomata, sugarcane continues to photosynthesise rapidly whereas wheat slows down. The main reason is that sugarcane:

    • A. Concentrates CO₂ around RuBisCO in bundle-sheath cells, limiting photorespiration
    • B. Opens its stomata only at night like cacti
    • C. Has no RuBisCO
    • D. Uses oxygen as its carbon source
  10. Q15. Pineapple and cacti open their stomata at night and store CO₂ as organic acids. The main advantage of this is:

    • A. Photosynthesis without light
    • B. Reduced water loss in hot, dry conditions
    • C. Avoiding the need for chlorophyll
    • D. Faster growth in shade
  11. Q16. In a greenhouse on a bright winter day, the factor most likely to limit photosynthesis is:

    • A. Low temperature
    • B. Chlorophyll content
    • C. Oxygen concentration
    • D. Light intensity
  12. Q17. In glycolysis, the step that uses ATP and commits glucose to the pathway is catalysed by phosphofructokinase. The product formed is:

    • A. Glyceraldehyde-3-phosphate
    • B. Glucose-6-phosphate
    • C. Pyruvate
    • D. Fructose-1,6-bisphosphate
  13. Q18. During glycolysis, a total of how many ATP molecules are produced (gross) and used per glucose?

    • A. 4 produced and 0 used
    • B. 6 produced and 2 used
    • C. 4 produced and 2 used
    • D. 2 produced and 2 used
  14. Q19. Why must yeast in an anaerobic fermenter convert pyruvate to ethanol rather than simply accumulating pyruvate?

    • A. To produce extra ATP from ethanol
    • B. To regenerate NAD⁺ so that glycolysis can continue
    • C. To make glucose from pyruvate
    • D. To release oxygen for the cell
  15. Q20. Aerobic respiration of one glucose yields about 38 ATP (textbook value), whereas lactic acid fermentation yields 2 ATP. How many times more ATP does aerobic respiration release per glucose?

    • A. 19
    • B. 38
    • C. 36
    • D. 17
  16. Q21. The enzyme complex that converts pyruvate to acetyl CoA requires several coenzymes. A deficiency of which vitamin impairs this step and causes beriberi?

    • A. Vitamin D
    • B. Thiamine (vitamin B₁)
    • C. Vitamin K
    • D. Vitamin C
  17. Q22. The Krebs cycle takes place in the:

    • A. Cytoplasm
    • B. Mitochondrial matrix
    • C. Inner mitochondrial membrane
    • D. Thylakoid lumen
  18. Q23. Per molecule of glucose, the Krebs cycle (two turns) directly produces:

    • A. 2 NADH, 2 FADH₂ and 2 ATP
    • B. 8 NADH, 2 FADH₂ and 4 ATP
    • C. 6 NADH, 2 FADH₂ and 2 ATP (GTP)
    • D. 3 NADH, 1 FADH₂ and 1 ATP
  19. Q24. Electrons from FADH₂ enter the electron transport chain at a lower energy level than electrons from NADH. As a result, FADH₂ yields:

    • A. More ATP than NADH
    • B. The same ATP as NADH
    • C. No ATP at all
    • D. About 2 ATP compared with about 3 for NADH
  20. Q25. Which component carries electrons between complex III and complex IV in the mitochondrial chain?

    • A. Ferredoxin
    • B. Cytochrome c
    • C. Coenzyme Q
    • D. NAD⁺
  21. Q26. The enzyme that makes ATP as protons flow back through the inner mitochondrial membrane is:

    • A. Hexokinase
    • B. RuBisCO
    • C. ATP synthase
    • D. ATPase of the sodium pump
  22. Q27. Carbohydrate has a respiratory quotient (RQ) of 1.0 and fat about 0.7. If an organism's measured RQ is 0.9, the most likely substrate mixture is:

    • A. An organic acid such as malic acid
    • B. Carbohydrate together with some fat or protein
    • C. Pure carbohydrate
    • D. Pure fat only
  23. Q28. Germinating seeds are placed in a respirometer with KOH to absorb CO₂. The coloured liquid in the capillary moves towards the seeds. This movement measures:

    • A. The water absorbed by the seeds
    • B. The volume of CO₂ released
    • C. The rate of photosynthesis
    • D. The volume of oxygen taken up
  24. Q29. Hydrolysis of ATP to ADP and inorganic phosphate releases energy mainly because:

    • A. Oxygen is consumed in the reaction
    • B. A peptide bond is broken
    • C. The products are more stable than ATP, with less repulsion between negative phosphate groups
    • D. ADP has more phosphate groups than ATP
  25. Q30. Stacks of thylakoids in a chloroplast are called grana. Their arrangement benefits photosynthesis mainly by:

    • A. Storing starch for the night
    • B. Containing the chloroplast DNA
    • C. Holding the Calvin cycle enzymes
    • D. Providing a large membrane surface for pigments and electron carriers